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Part 1: Document Description
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Citation |
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Title: |
Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR |
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Identification Number: |
doi:10.7910/DVN/B2PT24 |
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Distributor: |
Harvard Dataverse |
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Date of Distribution: |
2017-05-14 |
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Version: |
1 |
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Bibliographic Citation: |
Kamau, Everlyn; Nokes, David James, 2017, "Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR", https://doi.org/10.7910/DVN/B2PT24, Harvard Dataverse, V1 |
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Citation |
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Title: |
Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR |
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Identification Number: |
doi:10.7910/DVN/B2PT24 |
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Authoring Entity: |
Kamau, Everlyn (Kemri Wellcome Trust Research programme) |
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Nokes, David James (Epidemiology and Demography Department, Kenya Medical Research Institute (KEMRI) – Wellcome Trust Research Programme, Kilifi, Kenya School of Life Sciences and WIDER, University of Warwick, Coventry, United Kingdom) |
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Distributor: |
Harvard Dataverse |
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Access Authority: |
Kamau, Everlyn |
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Depositor: |
Kamau, Everlyn |
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Date of Deposit: |
2017-05-08 |
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Holdings Information: |
https://doi.org/10.7910/DVN/B2PT24 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Respiratory Syncytial Virus, Real-Time Mismatches, Probe |
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Abstract: |
<p>This dataset comprises of Nucleotide sequences used to match primers and probes, and for phylogenetic inferences. A total of 168 N and G gene sequences deposited in Genbank with accession numbers from KX775772 - KX775940. This data is freely available. In our analysis, we observed inconsistencies between two routine RSV diagnostic methods applied in our labs, one method utilizes immunofluorescence microscopy and other molecular probes, and set out to determine.</p> <p> Sequence data cleaning and contig assembly was done using Sequencher program v.5.1; sequence alignment done using MAFFT v.1.8; phylogenetic inferences done in MEGA v1.6; custom BLAST of the primers and probes done using geneious v8.1.8 </p> |
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Notes: |
<strong>Data Availability</strong> <p>All sequence data reported in this publication is freely available in Genbank with accession numbers from KX775772 - KX775940. </p> |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Notes: |
<a href="http://creativecommons.org/publicdomain/zero/1.0">CC0 1.0</a> |
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Other Study Description Materials |
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Label: |
KamauE_Sequence_Variation_jcv_2017.pdf |
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Notes: |
application/pdf |