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Part 1: Document Description
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Citation |
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Title: |
Replication Data for: Bioelectric Characterization of Senescing Human Keratinocytes |
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Identification Number: |
doi:10.7910/DVN/NEU8UO |
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Distributor: |
Harvard Dataverse |
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Date of Distribution: |
2024-09-24 |
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Version: |
2 |
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Bibliographic Citation: |
Sediqi, Hamid; Levin, Michael, 2024, "Replication Data for: Bioelectric Characterization of Senescing Human Keratinocytes", https://doi.org/10.7910/DVN/NEU8UO, Harvard Dataverse, V2 |
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Citation |
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Title: |
Replication Data for: Bioelectric Characterization of Senescing Human Keratinocytes |
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Identification Number: |
doi:10.7910/DVN/NEU8UO |
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Authoring Entity: |
Sediqi, Hamid (Tufts University) |
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Levin, Michael (Tufts University) |
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Distributor: |
Harvard Dataverse |
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Access Authority: |
Sediqi, Hamid |
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Depositor: |
Poirier, Julia E |
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Date of Deposit: |
2024-09-18 |
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Holdings Information: |
https://doi.org/10.7910/DVN/NEU8UO |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, aging, resting potential, bioelectric pattern, in vitro, cell culture |
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Abstract: |
Images are organized based on the figure they belong to. The 'Figure 1' folder contains Hoechst and BeRST images for each keratinocyte age. These images were used to quantify the bioelectric properties of keratinocytes across different ages. They are stitched images and have been labeled numerically based on the replicate number. The 'Figure 2' folder contains Beta-galactosidase and Hoechst images. These images were used to calculate the integrated density of the β-Gal signal per cell. Biological replicates are indicated by the numerical names of the folders for each age. The 'Figure 3' folder contains BeRST time-series images of keratinocytes at varying ages, taken pre- and post-treatment with Pinacidil. These images were used to determine the degree of responsiveness to drug treatments as well as the level of resilience. The 'Figure 4' folder contains spatial images used to compute Moran’s I calculations. The 'Figure 5' folder contains images of β-Gal and Hoechst. These images were used to calculate β-Gal activity, cell number, and chromatin condensation levels. The 'Figure 6' folder contains time-series images of cells pretreated with bioelectric-modulating conditions. These data were used to calculate the responsiveness of pretreated cells. The 'Figure 7' folder contains spatial images of pretreated keratinocytes used to compute Moran’s I autocorrelation. The 'SuppFig1' folder contains VF2.0 images at 600 nM and 50 nM concentrations. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Sediqi, H., and Levin, M. (2024), Bioelectric Characterization of Senescing Human Keratinocytes, OSF Preprints, awaiting moderator review |
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Identification Number: |
not yet assigned |
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Bibliographic Citation: |
Sediqi, H., and Levin, M. (2024), Bioelectric Characterization of Senescing Human Keratinocytes, OSF Preprints, awaiting moderator review |
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Citation |
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Title: |
Sediqi, H., and Levin, M. (2024), Bioelectric Characterization of Senescing Human Keratinocytes, PNAS Nexus, under review |
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Identification Number: |
not yet assigned |
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Bibliographic Citation: |
Sediqi, H., and Levin, M. (2024), Bioelectric Characterization of Senescing Human Keratinocytes, PNAS Nexus, under review |
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Label: |
Data_Compiled.zip |
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Text: |
Images are organized based on the figure they belong to. The Figure 1 folder contains Hoechst and BeRST images for each keratinocyte age. These images were used to quantify the bioelectric properties of keratinocytes across different ages. They are stitched images and have been labeled numerically based on replicate number. The Figure 2 folder contains Beta-galactosidase and Hoechst images. These images were used to calculate the integrated density of the β-Gal signal per cell. Biological replicates are indicated by the numerical folder names for each age. The Figure 3 folder contains BeRST time-series images of keratinocytes at varying ages, pre- and post-treatment with Pinacidil. These images were used to determine the degree of responsiveness to drug treatments as well as the level of resilience. The Figure 4 folder contains spatial images used to compute Moran's I calculations. The Figure 5 folder contains images of Beta-gal and Hoechst. These images were used to calculate β-Gal activity, cell number, and chromatin condensation levels. The Figure 6 folder contains time-series images of cells pretreated with bioelectric-modulating conditions. These data were used to calculate the responsiveness of pretreated cells. The Figure 7 folder contains spatial images of pretreated keratinocytes used to compute Moran's I autocorrelation. The SuppFig1 folder contains VF2.0 images at 600 nM and 50 nM concentrations. |
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Notes: |
application/zip |