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Part 1: Document Description
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Citation |
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Title: |
Placental miR-3940-3p is associated with maternal insulin resistance in late pregnancy |
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Identification Number: |
doi:10.7910/DVN/SSSL2D |
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Distributor: |
Harvard Dataverse |
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Date of Distribution: |
2021-03-26 |
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Version: |
1 |
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Bibliographic Citation: |
O'tierney-Ginn, Perrie Faye; Alvarado, Fernanda, 2021, "Placental miR-3940-3p is associated with maternal insulin resistance in late pregnancy", https://doi.org/10.7910/DVN/SSSL2D, Harvard Dataverse, V1 |
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Citation |
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Title: |
Placental miR-3940-3p is associated with maternal insulin resistance in late pregnancy |
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Identification Number: |
doi:10.7910/DVN/SSSL2D |
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Authoring Entity: |
O'tierney-Ginn, Perrie Faye (Tufts University) |
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Alvarado, Fernanda (Tufts University) |
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Distributor: |
Harvard Dataverse |
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Access Authority: |
O'tierney-Ginn, Perrie Faye |
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Depositor: |
O'tierney-Ginn, Perrie Faye |
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Date of Deposit: |
2021-03-17 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, placenta, miRNA, insulin resistance |
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Abstract: |
Context: An increase in maternal insulin resistance (IR) during pregnancy is essential for normal fetal growth. The mechanisms underlying this metabolic adaptation to pregnancy are poorly understood. Placenta factors are believed to instigate and maintain these metabolic changes, as IR decreases shortly after delivery. Methylation of placental gene loci that are common targets for miRNAs, are associated with maternal IR. Objective: We hypothesized that placental miRNAs targeting methylated loci are associated with maternal IR during late pregnancy. Methods: Placentas were collected from 132 elective cesarean sections and fasting maternal HOMA-IR at delivery was calculated. Placental miRNA expression was measured via small RNA-sequencing in a subset of 40 placentas selected by maternal pre-gravid BMI and neonatal adiposity. Five miRNAs that correlated with maternal HOMA-IR and were previously identified as targeting methylated genes were selected for validation in all 132 placenta samples via RT-qPCR. Multilinear regression was used to adjust for relevant clinical variables. Results: Median maternal age was 27.5 years, with a median pre-pregnancy BMI of 24.7 kg/m2, and median HOMA-IR of 2.9. Among the five selected miRNA, near delivery maternal HOMA-IR correlated with the placental expression of miRNA-371b-3p (r=0.25; p=0.008) and miRNA-3940-3p (r=0.32; p=0.0004) across the 132 individuals. After adjustment for confounding variables, placental miRNA-3940-3p expression remained significantly associated with HOMA-IR (β=0.16; p=0.03). Conclusion: Placental miRNA-3940-3p was associated with maternal IR in near delivery. This placental miRNA may have an autocrine or paracrine effect - regulating placental genes which play a role in modulating maternal IR. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Notes: |
CC0 Waiver |
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Other Study Description Materials |
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Label: |
SupplTable1_Alvarado 2021.docx |
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Text: |
Demographic characteristics of the small RNA sequencing subset (N=40) |
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Notes: |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |
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Label: |
SupplTable2_Alvarado 2021.docx |
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Placental miRNAs expression levels correlated with maternal HOMA-IR in late pregnancy (Pearson estimation and P-values shown) (columns A, B & C) and miRNA predicted to target genes nearest to CpG sites at which placental DNA methylation was associated with maternal Matsuda index (reciprocal to insulin resistance) in mid-pregnancy (Hivert et al., 2020; P-values from miRNA TargetScan emerging from list of genes identified) |
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Notes: |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |
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Label: |
SupplTable3_Alvarado 2021.docx |
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Primer information |
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Notes: |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |