Statistical analysis (GLMM) for: Implications of the existence of separated sexual forms of Opuntia robusta for plant defense hypotheses (doi:10.7910/DVN/USSQ3F)

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Statistical analysis (GLMM) for: Implications of the existence of separated sexual forms of Opuntia robusta for plant defense hypotheses

doi:10.7910/DVN/USSQ3F

Harvard Dataverse

2020-08-13

1

Janczur, Mariusz Krzysztof; Sandoval-Molina, Mario Alberto; González-Camarena, Emilio, 2020, "Statistical analysis (GLMM) for: Implications of the existence of separated sexual forms of Opuntia robusta for plant defense hypotheses", https://doi.org/10.7910/DVN/USSQ3F, Harvard Dataverse, V1, UNF:6:1olteIpxz9qqtQ6OSX72Xg== [fileUNF]

Statistical analysis (GLMM) for: Implications of the existence of separated sexual forms of Opuntia robusta for plant defense hypotheses

Separated sexual forms of Opuntia robusta: Statistical analysis (GLMM).

doi:10.7910/DVN/USSQ3F

Janczur, Mariusz Krzysztof (Universidad Autónoma del Estado de México)

Sandoval-Molina, Mario Alberto (Instituto de Ecología A.C., Red de Ecología Funcional. Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz 91070, México.)

González-Camarena, Emilio (Universidad Autónoma del Estado de México)

Sandoval-Molina, Mario Alberto

Janczur, Mariusz Krzysztof

Janczur, Mariusz Krzysztof

2020-08-09

180694

Harvard Dataverse

Janczur, Mariusz Krzysztof

Janczur, Mariusz Krzysztof

Janczur, Mariusz Krzysztof

2020-08-10

2020-08-09

https://doi.org/10.7910/DVN/USSQ3F

Medicine, Health and Life Sciences, Opuntia robusta, trioecious populations, plant sexual forms, female plants, hermaphrodite plants, secondary metabolites, 4-Hydroxybenzoic acid, Chlorogenic acid, Quercetin

Plant defense against herbivores

We analyze the effect of different variables on the concentrations of 4-Hydroxybenzoic acid (4-HBA), chlorogenic acid (CGA) and quercetin (QUE) in female and hermaphrodite individuals of Opuntia robusta from San Nicolas Tecoaso, Municipality of Singuilucan, Hidalgo State, Central Mexico, using general linear mixed models (GLMM). To analyze the effect of months and age on 4-HBA concentrations, we used the Gamma error distribution (GED) and the inverse link function. For the effect of months on CGA concentrations, we used GED and log link functions. For the effect of cladode age on CGA concentrations, GED and inverse link functions. For the effect of months on QUE concentration, GED and log link functions. For the effect of cladode age on QUE concentration, GED and inverse link function. For the models that related substances with cladode length, width and thickness, we used different models: for 4-HBA concentrations we used a GED with square root link function. For CGA concentrations, GED and identity link function, and for QUE concentrations, GED and inverse link function. To test the effect of cladodes order from soil on the concentrations of the three substances, we used GED and square root link function. To test the effect of the number of cladodes above a given cladode on the concentrations of the three substances, we used GED and inverse link function. We checked the goodness of fit of the linear models (GLMM) by plotting the standardized residuals against fitted values and by normal QQ‐plots, and revised the assumptions of the homoscedasticity, proper distribution used, and independence. We chose the best GLM model used in this study according to the lowest AIC (Akaike, 1974; Akaike, 1987).

2014-03-01-2017-06-30

2014-03-01-2017-06-30

Concentrations of secondary metabolites in cladodes of Opuntia robusta

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Not embargoed

There exist only one file containing data. The other file contains the results of the statistical analyses.

This is the complete data gathered during the part of the study concerning the production of secondary metabolites in Opuntia robusta.

As far as I know, the use of this data does not affect interests of any third party person or organization. It may affect only the authors of the data if not used in accordance of this Term of use.

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Mariusz Krzysztof Janczur majmx@interia.pl majmx@yahoo.com.mx majmx@biolevolmex.com

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I whereby declare that I financed the collection of the data uploaded here and participated in the fieldwork. Also I declare that I was the project owner.

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• Number of cases: 4

• No. of variables per record: 1

• Type of File: text/tab-separated-values

UNF:6:1olteIpxz9qqtQ6OSX72Xg==

Morphometric traits, cladode order counted from soil, number of cladodes above a given clacode, date of sampling, spine color (cladode age estimator) absorbanse (surface belowe the absorbance curve), calibrations equations, and concentration of 4-hydroxybenzoic acid (4-HBA), quercetine (QUE), salicilic acid (SAL), and chlorogenic acid (CGA) obtained from the calibration equations for females and hermafrodite cladodes

• Morphometrictraits,cladodeordercountedfromsoil,numberofcladodesaboveagivenclacode,dateofsampling, - Morphometrictraits,cladodeordercountedfromsoil,numberofcladodesaboveagivenclacode,dateofsampling,

f4022974 Location:

Variable Format: character

Notes: UNF:6:1olteIpxz9qqtQ6OSX72Xg==

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Supplementary Table S5.docx

Text:

We analyze the effect of different variables on the concentrations of 4-Hydroxybenzoic acid (4-HBA), chlorogenic acid (CGA) and quercetin (QUE) in female and hermaphrodite individuals of Opuntia robusta from San Nicolas Tecoaso, Municipality of Singuilucan, Hidalgo State, Central Mexico, using general linear mixed models (GLMM). To analyze the effect of months and age on 4-HBA concentrations, we used the Gamma error distribution (GED) and the inverse link function. For the effect of months on CGA concentrations, we used GED and log link functions. For the effect of cladode age on CGA concentrations, GED and inverse link functions. For the effect of months on QUE concentration, GED and log link functions. For the effect of cladode age on QUE concentration, GED and inverse link function. For the models that related substances with cladode length, width and thickness, we used different models: for 4-HBA concentrations we used a GED with square root link function. For CGA concentrations, GED and identity link function, and for QUE concentrations, GED and inverse link function. To test the effect of cladodes order from soil on the concentrations of the three substances, we used GED and square root link function. To test the effect of the number of cladodes above a given cladode on the concentrations of the three substances, we used GED and inverse link function. We checked the goodness of fit of the linear models (GLMM) by plotting the standardized residuals against fitted values and by normal QQ‐plots, and revised the assumptions of the homoscedasticity, proper distribution used, and independence. We chose the best GLM model used in this study according to the lowest AIC (Akaike, 1974; Akaike, 1987). (2020-08-09)

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