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Part 1: Document Description
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Citation |
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Title: |
Figure S5 Plasmids with two PaqCI target sites show potential sequential cleavage when trans oligo enhancer is added |
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Identification Number: |
doi:10.7910/DVN/XTQZXI |
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Distributor: |
Harvard Dataverse |
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Date of Distribution: |
2022-10-13 |
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Version: |
1 |
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Bibliographic Citation: |
Kennedy, Madison, 2022, "Figure S5 Plasmids with two PaqCI target sites show potential sequential cleavage when trans oligo enhancer is added", https://doi.org/10.7910/DVN/XTQZXI, Harvard Dataverse, V1 |
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Citation |
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Title: |
Figure S5 Plasmids with two PaqCI target sites show potential sequential cleavage when trans oligo enhancer is added |
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Identification Number: |
doi:10.7910/DVN/XTQZXI |
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Authoring Entity: |
Kennedy, Madison (Fred Hutchinson Cancer Research Center) |
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Distributor: |
Harvard Dataverse |
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Access Authority: |
Kennedy, Madison |
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Depositor: |
Kennedy, Madison |
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Date of Deposit: |
2022-10-05 |
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Holdings Information: |
https://doi.org/10.7910/DVN/XTQZXI |
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Study Scope |
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Keywords: |
Chemistry, Medicine, Health and Life Sciences, Chemistry, Medicine, Health and Life Sciences |
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Abstract: |
The reaction contained 1 ug substrate DNA, 2 units/ug PaqCI endonuclease and 40 nM/unit activating oligonucleotide in rCutSmart buffer at 37C. Reactions were quenched at the time points indicated above each lane. The mobilities of the super coil (SC) and the linear (Lin) forms of the plasmid are indicated in the middle. On the right of the gels is a cartoon of the stages of DNA degradation shown in the gel. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Label: |
FigureS5_headtoheadwitholigo.jpg |
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Text: |
Figure S5 - Time course digest of head-to-head plasmid with enhancing oligo |
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Notes: |
image/jpeg |
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Label: |
FigureS5_headtotailwitholigo.jpg |
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Text: |
Figure S5 - Time course digest of head-to-tail plasmid with enhancing oligo |
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Notes: |
image/jpeg |