Replication Data for: A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)

Version 1.6
Jaramillo, Angélica M; Londoño, Luis Fernando; Orozco, Juan Camilo; Patiño, Gelver; Belalcazar, John; Davrieux , Fabrice; Talsma, Elise, 2018, "Replication Data for: A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)", https://doi.org/10.7910/DVN/D6GU8U, Harvard Dataverse, V1, UNF:6:kvaFh8ql7M1WsmwTpNKaMQ== [fileUNF]
Learn about Data Citation Standards.
Dataset Metrics
155 Downloads
Description

The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC.


The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer.


According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment.

Subject Earth and Environmental Sciences; Agricultural Sciences
Keyword Manihot esculenta, Method comparison, Biofortification, All-trans-β-carotene, HPLC, Bland altman, Latin America and the Caribbean, Agrobiodiversity - AGBIO, HarvestPlus
Related Publication Jaramillo, Angélica M.; Londoño, Luis Fernando; Orozco, Juan Camilo; Patiño, Gelver; Belalcazar, John; Davrieux, Fabrice & Talsma, Elise F. (2018). A comparison study of five different methods to measure carotenoids in biofortified yellow cassava ( Manihot esculenta ). Plos One,13(12): e0209702.doi: 10.1371/journal.pone.0209702
Notes Cassava storage roots with low, media and high carotenoid levels
License/Data Use Agreement

Custom Dataset Terms

Filter by
1 to 4 of 4 Files
01. Dictionary.xls
MS Excel Spreadsheet - 33.0 KB
Published Dec 5, 2018
35 Downloads
MD5: f2a435d503039c795c2cf8da496b6d34
Documentation
02. Method comparison study.tab
Tabular Data - 3.2 KB
Published Dec 5, 2018
48 Downloads
9 Variables, 60 Observations UNF:6:R7oEBPZxgpr6YNWnOK4LSQ==
Data
Preview "02. Method comparison study.tab" Preview "02. Method comparison study.tab"
03. iCheck comparison study.tab
Tabular Data - 496 B
Published Dec 5, 2018
36 Downloads
3 Variables, 20 Observations UNF:6:nOlDVVzdvnGvdH6HuihJ5Q==
Data
Preview "03. iCheck comparison study.tab" Preview "03. iCheck comparison study.tab"
04. Retention study.tab
Tabular Data - 488 B
Published Dec 5, 2018
36 Downloads
7 Variables, 10 Observations UNF:6:N3a2dR5tTGvc9bGyKYFbLw==
Data
Preview "04. Retention study.tab" Preview "04. Retention study.tab"
Edit File

This file has already been deleted (or replaced) in the current version. It may not be edited.

Restrict Access

Restricting limits access to published files. People who want to use the restricted files can request access by default. If you disable request access, you must add information about access to the Terms of Access field.

Learn about restricting files and dataset access in the User Guide.

Enable access request
You must enable request access or add terms of access to restrict file access.
Save Changes
Edit Embargo

The selected file or files have already been published. Contact an administrator to change the embargo date or reason of the file or files.
Edit Retention Period

The selected file or files have already been published. Contact an administrator to change the retention period date or reason of the file or files.
Delete Files

The file will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select one or more files.

Share Dataset

Share this dataset on your favorite social media networks.

Continue
Dataset Citations

Citations for this dataset are retrieved from Crossref via DataCite using Make Data Count standards. For more information about dataset metrics, please refer to the User Guide.

Sorry, no citations were found.
Inaccessible Files Selected

The selected file(s) may not be downloaded because you have not been granted access or the file(s) have a retention period that has expired or the files can only be transferred via Globus.

Ineligible Files Selected

The selected file(s) may not be transferred because you have not been granted access or the file(s) have a retention period that has expired or the files are not Globus accessible.

Download Options

The files selected are too large to download as a ZIP.

You can select individual files that are below the 15.0 GB download limit from the files table, or use the Data Access API for programmatic access to the files.

Select File(s)

Please select a file or files to be downloaded.

Inaccessible Files Selected

The selected file(s) may not be downloaded because you have not been granted access or the file(s) have a retention period that has expired.

Click Continue to download the files you have access to download.

Ineligible Files Selected

Some file(s) cannot be transferred. (They are restricted, embargoed, with an expired retention period, or not Globus accessible.)

Click Continue to transfer the elligible files.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Unpublished Dataset Preview URL

Preview URL can only be used with unpublished versions of datasets.

Unpublished Dataset Preview URL

Are you sure you want to disable the Preview URL? If you have shared the Preview URL with others they will no longer be able to use it to access your unpublished dataset.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Compute

This dataset contains restricted files you may not compute on because you have not been granted access.

Deaccession Dataset

Are you sure you want to deaccession? This is permanent and the selected version(s) will no longer be viewable by the public.

Deaccession Dataset

Are you sure you want to deaccession this dataset? This is permanent an it will no longer be viewable by the public.

Version Differences Details

Please select two versions to view the differences.

Version Differences Details
 
Version:
Last Updated:
Version:
Last Updated:
Select File(s)

Please select a file or files for access request.

Select File(s)

Embargoed files cannot be accessed. Please select an unembargoed file or files for your access request.

Edit Tags

Select existing file tags or create new tags to describe your files. Each file can have more than one tag.

Request Access

  You need to Sign Up or Log In to request access.

Dataset Terms

Please confirm and/or complete the information needed below in order to request access to files in this dataset.

This dataset is made available under the following terms. Please confirm and/or complete the information needed below in order to continue.

Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation shown on the dataset page.

Custom Dataset Terms - the following Custom Dataset Terms have been defined for this dataset.

Creative Commons License
These data and documents are licensed under a Creative Commons Attribution 4.0 International license. You may copy, distribute and transmit the data as long as you acknowledge the source through proper data citation.

Disclaimer

Whilst utmost care has been taken by CIAT and data authors while collecting and compiling the data, the data is however offered "as is" with no express or implied warranty. In no event shall the data authors, CIAT, or relevant funding agencies be liable for any actual, incidental or consequential damages arising from use of the data.

By using the CIAT Dataverse, the user expressly acknowledges that the Data may contain some nonconformities, defects, or errors. No warranty is given that the data will meet the user's needs or expectations or that all nonconformities, defects, or errors can or will be corrected.

The user should always verify actual data; therefore the user bears all responsibility in determining whether the data is fit for the user’s intended use. The user of the data should use the related publications as a baseline for their analysis whenever possible. Doing so will be an added safeguard against misinterpretation of the data. Related publications are listed in the metadata section of the Dataverse study.

Whilst utmost care has been taken by CIAT and data authors while collecting and compiling the data, the data is however offered "as is" with no express or implied warranty. In no event shall the data authors, CIAT, or relevant funding agencies be liable for any actual, incidental or consequential damages arising from use of the data.

By using the CIAT Dataverse, the user expressly acknowledges that the Data may contain some nonconformities, defects, or errors. No warranty is given that the data will meet the user's needs or expectations or that all nonconformities, defects, or errors can or will be corrected.

The user should always verify actual data; therefore the user bears all responsibility in determining whether the data is fit for the user’s intended use. The user of the data should use the related publications as a baseline for their analysis whenever possible. Doing so will be an added safeguard against misinterpretation of the data. Related publications are listed in the metadata section of the Dataverse study.
Preview Guestbook

Upon downloading files the guestbook asks for the following information.

Account Information

Package File Download

Use the Download URL in a Wget command or a download manager to download this package file. Download via web browser is not recommended. User Guide - Downloading a Dataverse Package via URL

https://qa.dataverse.org/api/access/datafile/

Compute Batch
Clear Batch
Dataset Persistent Identifier Change Compute Batch
Compute Batch
Submit for Review

You will not be able to make changes to this dataset while it is in review.

Publish Dataset

Are you sure you want to republish this dataset?

By default datasets are published with the CC0-“Public Domain Dedication” waiver. Learn more about the CC0 waiver here.

To publish with custom Terms of Use, click the Cancel button and go to the Terms tab for this dataset.

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until CIAT - International Center for Tropical Agriculture Dataverse is published by its administrator.

Publish Dataset

This dataset cannot be published until CIAT - International Center for Tropical Agriculture Dataverse and Harvard Dataverse are published.

Return to Author

Return this dataset to contributor for modification. The reason for return entered below will be sent by email to the author.

Curation Status History
StatusDateAssigner
No records found.
Add/Edit a Version Note
Styled Citation