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Kennedy, Madison, 2023, "Figure 9 DNA pre-bound at all four sites in the PaqCI tetramer is cut sequentially", https://doi.org/10.7910/DVN/2SZXFW, Harvard Dataverse, V1
Panel a. Cleavage of a four-site plasmid substrate either pre-bound or not pre-bound with a 1:1 enzyme-binding-site to DNA-sites ratio. The pre-bound reaction contained 1 ug substrate DNA (37 nM sites) and 4.3 units (37 nM) of PaqCI per ug DNA per 50 ul rCutSmart buffer lacking Mg++ ions and enzyme was allowed to bind for 15 minutes at 37C. An aliq... |
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MD5: 07cb98f77b8a1737a280db96ee00bdf5
Figure 9a - 1:1 Pre-Bind 15 min digest of head-to-tail plasmid |
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MD5: 4e88e9a8109eb8a4da41d43c86822bd7
Figure 9a - 1:1 No Pre-Binding digest of head-to-tail plasmid |
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MD5: 891b5d3d4cc8525992b7d4678ade11bd
Figure 9b - 2:1 Enzyme:Sites digest of head-to-tail plasmid |
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MD5: 4aa4f617ddaaa46f44a3675ebdf4577b
Figure 9b - 4:1 Enzyme:Sites digest of head-to-tail plasmid |
Kennedy, Madison, 2023, "Figure 8 Time-dependent digestion of plasmids with four PaqCI target sites indicate a sequential cleavage at individual sites", https://doi.org/10.7910/DVN/QEEZ7Y, Harvard Dataverse, V1
Panel a. Cleavage of a head-to-head four-site substrate with and without an activator added in trans. The no activator reaction contained 1 ug substrate DNA (37 nM sites) and 2 units (17.2 nM) of PaqCI per ug DNA in 50 ul rCutSmart buffer at 37C. The activator reaction had the same substrate and PaqCI enzyme together with 80 nM activating oligodupl... |
Feb 3, 2023 -
Figure 8 Time-dependent digestion of plasmids with four PaqCI target sites indicate a sequential cleavage at individual sites
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MD5: 4441b9122f9142281ef7e6fd50122ac5
Figure 8a - time course digest of head-to-head plasmid with activator |
Feb 3, 2023 -
Figure 8 Time-dependent digestion of plasmids with four PaqCI target sites indicate a sequential cleavage at individual sites
JPEG Image - 24.8 KB -
MD5: 47e4060199b48accf0c185ae0f4e0ee2
Figure 8a - time course digest of head-to-head plasmid without activator |
Feb 3, 2023 -
Figure 8 Time-dependent digestion of plasmids with four PaqCI target sites indicate a sequential cleavage at individual sites
JPEG Image - 31.0 KB -
MD5: f4e44e5d5ca94e022eabc9eea869913f
Figure 8b - time course digest of head-to-tail plasmid with activator |
Feb 3, 2023 -
Figure 8 Time-dependent digestion of plasmids with four PaqCI target sites indicate a sequential cleavage at individual sites
JPEG Image - 21.5 KB -
MD5: a303de3ee097051483d006b059ed1066
Figure 8b - time course digest of head-to-tail plasmid without activator |
